A nanovehicle developed for treating deep-seated bacteria using low-dose X-ray.

摘要: Abstract Many non-antibiotic strategies, such as photocatalysis and photodynamic therapy, have been proposed to inhibit and/or kill bacteria. However, these approaches still drawbacks insufficient bacterial specificity the limited penetration depth of ultraviolet near-infrared light. To overcome limitations, we developed a bacteria-specific anti-bacterial technique via using low-dose X-ray. Graphene oxide quantum dots (GQDs, multifunctional vehicle) conjugated with vancomycin (Van, bacteria-targeting ligand) were assembled Protoporphyrin IX (PpIX, photo/radiation sensitizer) yield novel Van-GQDs/PpIX complex that specifically attached Escherichia coli efficiently generated intracellular reactive oxygen species following X-ray activation. Delivery GQDs increased PpIX/Van ratio in target cell, damaged cell wall, enhanced X-ray-induced PpIX Hence, this approach allowed for use activate exert its bactericidal effects on without damaging normal cells. Furthermore, E. did not develop resistance at least 7 rounds repeated administration during one week. Thus, vehicle exhibiting X-ray-triggered toxicity is promising alternative antibiotics treating serious infections occurring deep-seated tissues/organs (e.g., osteomyelitis peritonitis). Statements Significance Administration most common treatment modality infections. some cases, patient attributes age, health, tolerance do allow high-dose antibiotics. In addition, bacteria because improper long-term agents. Therefore, strategies treat deeply situated infections, osteomyelitis, are urgently needed avoiding amputation. date, several approaches, Ag nanoparticles, graphene-based materials, photocatalysis, therapy major challenges photochemical light source, remain problems, nanovehicle exerted toxic activation by penetrative X-ray, As such, it realizes route