Investigating the BSA protein adsorption and bacterial adhesion of Al-alloy surfaces after creating a hierarchical (micro/nano) superhydrophobic structure.

摘要: Bacterial adhesion and subsequent biofilm formation on metals such as aluminum (Al) alloys lead to serious issues in biomedical industrial fields from both an economical health perspective. Here, we showed that a careful manipulation of Al surface characteristics via facile two-steps superhydrophobic modification can provide not only biocompatibility ability control protein adsorption bacterial adhesion, but also address the issue apparent long-term toxicity Al-alloys. To find out roles characteristics, microbial formation, surfaces were systematically characterized by SEM, EDX, XPS, AFM, FTIR, water contact angle (WCA) goniometry, free energy (SFE) measurement, MTT, Bradford, Lowry microtiter plate assays flow-cytometry potentiostat analyses. Results WCA SFE changed 70° 163° 36.3 0.13 mN m(-1) , respectively. The stable durable led substantial reduction static/dynamic BSA adsorption. effect treatment was analyzed using three different bacteria Pseudomonas aeruginosa, Staphylococcus epidermidis, aureus. assay flow cytometry analysis could substantially reduce this biofouling resistance is independent bacterium type. An excellent cell viability after exposure HeLa cells waters incubated with modified samples observed. Finally, corrosion rate reduced sharply 856.6 0.119 MPY modifications, which inhibition property. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2220-2233, 2016.