Regulation of nerve growth and patterning by cell surface protein disulphide isomerase

摘要: Contact repulsion of growing axons is an essential mechanism for spinal nerve patterning. In birds and mammals the embryonic somites generate a linear series impenetrable barriers, forcing axon growth cones to traverse one half each somite as they extend towards their body targets. This study shows that protein disulphide isomerase provides key component these mediating contact at cell surface in half-somites. Repulsion reduced both vivo vitro by range methods inhibit enzyme activity. The activity critical initiating nitric oxide/S-nitrosylation-dependent signal transduction pathway regulates cone cytoskeleton. Rat forebrain grey matter extracts contain similar activity, expressed cultured human astrocytic cells rat cortical astrocytes. We suggest this system co-opted brain counteract regulate aberrant terminal growth.