Insufficient fumarase contributes to hypertension by an imbalance of redox metabolism in Dahl salt-sensitive rats.

摘要: Fumarase insufficiencies can increase reactive oxygen species (ROS). This study will further dissect the imbalance of redox metabolism and mechanism ROS production using proteomic technology in fumarase knockdown HK-2 cells. The contribution was confirmed by supplementation fumarate malate Dahl salt-sensitive rats. Proteomic analysis indicated that cells changed expression or activity NADPH oxidase (NOX), mitochondrial respiratory chain Complex I III, ATP synthase subunits, α-oxoglutarate dehydrogenase (OGDH). Meanwhile, activities key antioxidant enzymes, including glucose-6-phosphate dehydrogenase, 6-phosphogluconate glutathione reductase, peroxidase, S-transferase, increased significantly. apparent activation defense appeared insufficient as GSH/GSSG ratio were decreased rats exhibited changes similar to with knockdown. Supplementation decreased, respectively, blood pressure H2O2 malondialdehyde These results regulating NOX, ATPase alpha, OGDH metabolism, which may be one main reasons for hypertension. Malate a potentially effective drug prevention treatment