Wnt antagonist, secreted frizzled-related protein 1, is involved in prenatal skeletal muscle development and is a target of miRNA-1/206 in pigs

摘要: The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. Secreted frizzled-related proteins (SFRPs), such as SFRP1, function inhibitors signaling. MicroRNA-1/206(miRNA-1/206) specifically expressed play a critical role myogenesis. miRNA-mRNA profiles bioinformatics study suggested that SFRP1 gene was potentially regulated by miRNA-1/206 porcine To understand swine myogenesis, we first predicted targets with TargetScan PicTar programs, analyzed molecular characterization gene. We performed temporal-spatial expression analysis mRNA miRNA-206 Tongcheng pigs (a Chinese indigenous breed) quantitative real-time polymerase chain reaction, conducted co-expression analyses miRNA-1/206. Subsequently, interaction between validated via dual luciferase Western blot assays. to be target level highly varied across numerous pig tissues it down-regulated significantly higher embryonic compared postnatal muscle, whereas miR-206 showed inverse pattern expression. A significant negative correlation observed miR-1/206 development (p <0.05). Dual assay Western-blot results demonstrated iliac endothelial cells. Our indicate affects These findings increase understanding biological functions regulation mammals.