Induction and persistence of Mx protein in tissues, blood and plasma of Atlantic salmon parr, Salmo salar, injected with poly I:C.

摘要: Abstract The expression of Mx transcripts and protein was monitored at weekly intervals for 7 weeks, by qRT-PCR immunohistochemistry, in the kidney, liver, gill blood Atlantic salmon parr following injection poly I:C. Elevated levels compared to PBS injected control fish were found tissues week 1. Background then up 7, with exception 4 when high again I:C as well fish. Immunostaining liver higher than from weeks 1–4, but little staining both treated thereafter. Blood monocytes stained consistently all fish, suggesting that this leucocyte type constitutively expressed protein. From 2–4, lymphocytes groups consistency decreased 5–7. Staining neutrophils also inconsistent. Western blots plasma showed an immunoreactive band 76 kDa typical Semi-quantitative measurements dot have controls on 1–4 very low results indicate induction interferon response I:C, maintain elevated tissues, leucocytes about 4 weeks. Production appears be constitutive, though production less consistent.