Improperly folded green fluorescent protein is secreted via a non-classical pathway.

摘要: The green fluorescent protein is a cytosolic frequently used as molecular tag to study localization in intact cells. We discovered that this secreted into the medium by several but not all cell lines through non-classical secretory pathway insensitive brefeldin A. Green efficiently Chinese hamster ovary cells, with 60% of synthesized proteins over 8 hours. This sensitive changes temperature factors serum or chemicals known affect other secretion pathways. Fluorescence observed cells expressing protein, indicating some must be fully folded cytosol. However, and therefore properly. Furthermore, cellular fluorescence does change 6 hours whereas significant proportion secreted. Thus, nascent either correctly incorrectly, improperly molecules can exported. Non-classical might route which remove an excess folded, proteins.