Optimization of bio-hydrogen production from biodiesel wastes by Klebsiella pneumoniae.

摘要: Biodiesel wastes containing glycerol were utilized by Klebsiella pneumoniae DSM 2026 to produce hydrogen. The optimization of medium components was performed using both Plackett-Burman and uniform design methods. Using the design, glycerol, yeast extract, NH(4)Cl, KCl CaCl2 found be most important components, which further investigated second-order polynomial stepwise regression analysis. optimized 20.4 g.L(-1) 5.7 KCl, 13.8 1.5 CaCl(2) 3.0 extract resulted in 5.0-fold increased level hydrogen (57.6 mL/50 mL medium) production compared initial (11.6 after 24 h fermentation condition (pH, temperature inoculum) also conducted. When strain grew under optimal a 5-L stirred tank bioreactor for batch production, yield reached 0.53 mol/mol 117.8 mmol/L, respectively. maximum evolution rate 17.8 mmol/(L.h). Furthermore, 1,3-propanediol (6.7 g.L(-1)) obtained from liquid as by-product.