Growth, nutrient consumption, and end-product accumulation in Sf-9 and BTI-EAA insect cell cultures: insights into growth limitation and metabolism.

摘要: Growth, nutrient consumption, and end-product accumulation were quantitated in shake-flask cultures of two insect cell lines, Sf-9 BTI-EAA, three different serum-supplemented media. Per consumption or production rates calculated for most medium components analyzed. Glucose was growth-limiting TNM-FH the important single source organic-C cells all cultures. Cells utilized fructose maltose but not sucrose. alpha-Ketoglutarate malate contributed significantly to carbon budget TNM-FH. Lactate generally did accumulate during growth. Most amino acids consumed by cells, with exception alanine which produced. appeared be present adequate supply Glutamate rapidly acids, followed closely glutamine. Alanine correlated glucose consumption. In cultures, ammonia accumulated only slightly at as long medium, uric acid detectable end growth stationary phase. Added up a concentration 10 mM affect either line. Ammonia lactate may less importance limiting than mammalian A hypothetical outline major metabolic pathways cultured is presented on basis information obtained here literature.