Differentiation and activity of mast cells following immunization in cultures of lymph-node cells.

摘要: An extensive clonal differentiation into mast cells from primitive blast cell precursors occurred when lymph node obtained mice immunized with horse serum were cultured on mouse embryonic skin monolayers. Horse was always present in the culture as a constituent of nutritional medium. Mast developed to lesser extent also cultures prepared non-immunized mice. However, clear difference cell-granule ultrastructure and histamine content noted between two. In nodes granules tiny uniform size staining density; whereas immune larger non-uniform density, intragranular organization manifested alterations various forms. The intracellular per 10(6) about equal both cultures. much more free (per cells) gradually accumulated cells, indicating higher rates synthesis release histamine. readily degranulated by heat-inactivated (IgG1) sera used donors cells. 92% 80% released. degranulation accompanied an immediate (albeit reversible) response fibroblast monolayer. A shift well-stretched cytoplasm fibroblasts opened numerous 'window' over whole survived process could be maintained further Moreover, they capable repeated degranulation, releasing 50% their histamine, even after four cycles performed 7 days' period culture. No cytotoxic effect culture, 3 days seemed than undergranulated control cultures--suggesting intensified rate synthesis.