A system for the continuous directed evolution of biomolecules
作者： Kevin M. Esvelt , Jacob C. Carlson , David R. Liu
DOI: 10.1038/NATURE09929
关键词:
摘要: … PACE achieves continuous selection by linking the desired activity to the production of infectious progeny phage containing the evolving gene(s). Phage infection requires protein III (pIII…
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Christopher Avoigt, Stuart Kauffman, Zhen-Gang Wang, Rational evolutionary design: The theory of in vitro protein evolution Advances in Protein Chemistry. ,vol. 55, pp. 79- 160 ,(2001) , 10.1016/S0065-3233(01)55003-2
Atze T. Das, Xue Zhou, Monique Vink, Bep Klaver, Koen Verhoef, Giuseppe Marzio, Ben Berkhout, Viral Evolution as a Tool to Improve the Tetracycline-regulated Gene Expression System Journal of Biological Chemistry. ,vol. 279, pp. 18776- 18782 ,(2004) , 10.1074/JBC.M313895200
Daniel G Gibson, Lei Young, Ray-Yuan Chuang, J Craig Venter, Clyde A Hutchison, Hamilton O Smith, Enzymatic assembly of DNA molecules up to several hundred kilobases Nature Methods. ,vol. 6, pp. 343- 345 ,(2009) , 10.1038/NMETH.1318
J. W. Drake, A constant rate of spontaneous mutation in DNA-based microbes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 7160- 7164 ,(1991) , 10.1073/PNAS.88.16.7160
John N. Davis, Anthony N. van den Pol, Viral Mutagenesis as a Means for Generating Novel Proteins Journal of Virology. ,vol. 84, pp. 1625- 1630 ,(2010) , 10.1128/JVI.01747-09
Ilia E. Ichetovkin, Georgi Abramochkin, Thomas E. Shrader, Substrate Recognition by the Leucyl/Phenylalanyl-tRNA-protein Transferase CONSERVATION WITHIN THE ENZYME FAMILY AND LOCALIZATION TO THE TRYPSIN-RESISTANT DOMAIN Journal of Biological Chemistry. ,vol. 272, pp. 33009- 33014 ,(1997) , 10.1074/JBC.272.52.33009
Diane Imburgio, Minqing Rong, Kaiyu Ma, William T. McAllister, Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry. ,vol. 39, pp. 10419- 10430 ,(2000) , 10.1021/BI000365W
Craig T. Martin, Joseph E. Coleman, Kinetic analysis of T7 RNA polymerase-promoter interactions with small synthetic promoters. Biochemistry. ,vol. 26, pp. 2690- 2696 ,(1987) , 10.1021/BI00384A006
T. Opperman, S. Murli, B. T. Smith, G. C. Walker, A model for a umuDC-dependent prokaryotic DNA damage checkpoint. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 9218- 9223 ,(1999) , 10.1073/PNAS.96.16.9218
M. Camps, J. Naukkarinen, B. P. Johnson, L. A. Loeb, Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 9727- 9732 ,(2003) , 10.1073/PNAS.1333928100