Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors

摘要: We exploited, optimized, and developed various microscopy techniques for analyzing living matter at cellular molecular levels. In particular, we a toolbox single particle tracking (SPT) of membrane receptors their ligands, suitable also relatively fast single-pass receptors; this is based on chemical tagging recombinant proteins, TIRF microscopy, automatized analysis trajectories. are now extending it to simultaneous visualization two moieties. The superresolved localization technique allowed functions, interactions stoichiometry (pro)neurotrophin p75NTR TrkA ligands in cells, with particular attention some existing or used mutants. after treatments drugs unraveled mode action the first steps sundry signaling pathways.