Neoglycoproteins as carbohydrate antigens: synthesis, analysis, and polyclonal antibody response.
作者： Márta Kerékgyártó , Anikó Fekete , Zoltán Szurmai , János Kerékgyártó , László Takács
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摘要: The analysis and polyclonal antibody response for newly synthesized maltose-BSA conjugate neoglycoproteins is described. In this first proof of concept study, a simple carbohydrate antigen, maltose, was linked to BSA by reductive amination. An aglycone spacer utilized conserve the intact annular maltose structure promote accessibility immunogen hapten during immunization. were investigated CGE number conjugated residues determined MALDI-TOF MS. then evaluated immunization BALB/c mice tested ELISA as evidence presence sugar-containing epitope-specific antibodies. Selective binding demonstrated with different (low high) glycosylation degrees suggesting possible use approach generate Moreover, not inhibited or other carbohydrates confirm neoglycoprotein-specific
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