Adipose Differentiation Related Protein (ADRP) Expressed in Transfected COS-7 Cells Selectively Stimulates Long Chain Fatty Acid Uptake
作者： Jun Gao , Ginette Serrero
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摘要: Adipose differentiation related protein (ADRP) is a 50-kDa novel cloned from mouse 1246 adipocyte cDNA library, rapidly induced during differentiation. We have examined ADRP function, and we show here that facilitates fatty acid uptake in COS cells transfected with cDNA. demonstrate of long chain acids was significantly stimulated time-dependent fashion ADRP-expressing COS-7 compared empty vector-transfected control cells. Oleic velocity increased dose-dependent manner The transport Km 0.051 microM, Vmax 57.97 pmol/10(5) cells/min cells, 0.093 microM 20.13 oleate measured at 4 degrees C only 10% 37 C. also palmitate arachidonate but had no effect on medium such as octanoic glucose. These data suggest specifically enhances by increasing the initial rate provide information about function saturable component for acids.
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