Light-Dependent Hydrogen Evolution by Scenedesmus* **

摘要: The effect of glucose and the uncoupler Cl-CCP upon hydrogen production was studied in adapted cells Scenedesmus obliquus D3. at 10-5M concentration completely inhibited evolution H2 dark increased apparent rate light. At Cl-CCP, photosynthesis photoreduction by anaerobically algae were only temporarily inhibited; O2 reappeared after approximately 1 hr illumination if CO2 present. Increasing to 5 x led a maximum photohydrogen fully evolution, evolution. accompanied release varying amounts light, as well dark. Dark stimulated Cl-CCP. light adding autotrophically grown or growing heterotrophically with glucose; starvation had an opposite effect. Adapted released 14CO2 from 3 and/or 4 position specifically labeled glucose, indicating that degradation occurred via Embden-Meyerhof pathway. amount same either continuous short periods followed darkness. mutant No. 11, which is unable evolve not its capacity These data indicate depends organic material does require free photosystem II.