An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection.

摘要: Purified alpha-bungarotoxin was isolated by chromatography and made radioactive with tritium ([3H]acetamidino-alpha-bungarotoxin). Infusions of [3H]alpha-bungarotoxin alone or preceded tubocurarine atropine were given into the third ventricle. 2. 12, 24 h after injection brains prepared for autoradiography. Injections (radioinert) in buffer, [3H]parathyroid hormone served as controls. The various patterns labeling suggest presence nicotinic-cholinergic neurons within arcuate basolateral regions hypothalamus including supraoptic suprachiasmatic nuclei and, addition, central nucleus amygdala.