Detection of Mycobacterium avium subspecies avium in formalin-fixed, paraffin-embedded tissues of captive exotic birds using polymerase chain reaction.

摘要: A presumptive diagnosis of avian tuberculosis can be made when characteristic histologie lesions and acid-fast bacilli are observed in tissue samples. However, a definitive requires isolation identification the causative organism, process that take several weeks to complete. The purpose study was determine whether formalin-fixed, paraffin-embedded archival tissues could tested by polymerase chain reaction (PCR) reliably rapidly diagnose tuberculosis. Tissues were examined from both cases captive exotic birds obtained over 14-yr period (1983-1997). chosen consisted had with bacilli. primers used for PCR amplified 180 base-pair fragment 16S ribosomal RNA, sequence specific Mycobacterium avium subsp. M. paratuberculosis. If detected sample, it presumed a. organism being detected. This 26 97 samples (27%). Some negative results may explained any factors adversely affect nucleic acid integrity, particularly prolonged fixation formalin. Of 17 culture positive known have been fixed formalin <4 wk, 11 (65%). findings this show rapid indicator presence tissues. relatively low detection rate test demonstrated sample set limit its practical use as diagnostic tool.