Nanostructured silicate substituted calcium phosphate (NanoSiCaPs) nanoparticles - Efficient calcium phosphate based non-viral gene delivery systems.
作者： Sudhanshu Shekhar , Abhijit Roy , Daeho Hong , Prashant N. Kumta
DOI: 10.1016/J.MSEC.2016.06.076
关键词:
摘要: Abstract Nanostructured ceramic particles, particularly, nanoparticles of calcium phosphate (CaP) remain an attractive option among the various types non-viral gene delivery vectors studied because their safety, biocompatibility, biodegradability, and ease handling as well adsorptive capacity for DNA. We have accordingly developed enhanced version nanostructured phosphates (NanoCaPs), by substituting known amounts silicate in hydroxyapatite (HA) lattice (NanoSiCaPs). Results indicate that addition to excellent transfection levels exhibited un-substituted NanoCaPs alone vitro, additional 20–50% increase is observed containing 8.3–50 mol% aptly called NanoSiCaPs, owing its rapid dissolution properties enabling escaping lysosomal degradation. However, high substitution (> 50 mol%) resulted a drastic decline synthesized deviated far from characteristic phase formed evidenced materials characterization results.
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