CbrAB-dependent regulation of pcnB, a poly(A) polymerase gene involved in polyadenylation of RNA in Pseudomonas fluorescens.
作者： Xue-Xian Zhang , Yun-Hao Liu , Paul B. Rainey
DOI: 10.1111/J.1462-2920.2010.02228.X
关键词:
摘要: CbrB is a global sigma(54)-dependent regulator required for nutrient acquisition in Pseudomonas. Located downstream of cbrB on the Pseudomonas fluorescens SBW25 chromosome pcnB, putative poly(A) polymerase gene. Presence sigma(54) promoter intergenic region and pcnB led to hypothesis that regulates expression manner. Here we show transcription dependent. However, 5'-RACE analysis transcript using primers located coding shows starts immediately upstream ATG site at sigma(70)-like promoter. Deletion caused approximately 80% decrease ployadenylated 23S rRNA; growth mutant was compromised range laboratory media sugar beet seedlings. Further confirmed existence predicted Genetic showed drives crcZ, homologue recently described small RNA from aeruginosa, CbrB-dependent Taken together, our data both crcZ are part regulon. Moreover, draw further attention central regulatory role provides link between mRNA degradation cellular catabolism.
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