Precision-engineered reporter cell lines reveal ABCG2 regulation in live lung cancer cells.

摘要: Expression of the ABCG2 multidrug transporter is a marker cancer stem cells and predictor recurrent malignant disease. Understanding how human expression modulated by pharmacotherapy crucial in guiding therapeutic recommendations may aid rational drug development. Genome edited reporter are useful investigating gene regulation visualizing protein activity live but require precise targeting to preserve native regulatory regions. Here, we describe fluorescent assay that allows noninvasive assessment lung adenocarcinoma cells. Using CRISPR-Cas9 editing coupled with homology-directed repair, targeted an EGFP coding sequence translational start site ABCG2, generating knock-out situ tagged engineered cell lines, show upregulated number anti-cancer medications, HDAC inhibitors, hypoxia-mimicking agents glucocorticoids, supporting model which under control general stress response. To our knowledge, this first description system designed follow endogenous ABC The information gained guide therapy design.