Effect of the transducer's surface pre-treatment on SPR aptasensor development

摘要: Abstract The properties of a transducer's surface are crucial in biosensor development since they strongly condition the chemical routes applicable for bioreceptor incorporation and subsequent physical blocking. In this way, (bio)engineering should provide specificity against target choice minimal non-specific adsorption non-target components onto active area sensor. objective work was to study effect different treatments on SPR sensor functionalization optical performance. We used as model well-known thrombin-binding aptamer (TBA), which incorporated via two paths: direct selfassembly thiolated TBA affinity capture biotinylated by physisorbed neutravidin. Our results indicate that chip pre-treatment with oxygen plasma produced better transducers than immersion organic solvents, piranha or KOH solution, independently biofunctionalization strategy aptasensor production.