Cloning and Sequence Analysis of the hemB Gene of Rhodothermus marinus
作者： Katrín Gudmundsdóttir , Eiríkur Sigurdsson , Sigrídur I. Thorbjarnardóttir , Gudmundur Eggertsson
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摘要: A Rhodothermus marinus gene, hemB, coding for 5-aminolevulinic acid (ALA) dehydratase (ALAD) has been cloned and sequenced. The reading frame of the hemB gene is 1020 base pairs encoding a protein 340 amino acids with calculated molecular mass 37.4 kDa. sequence shows homology eubacterial eukaryotic ALA dehydratases. putative metal-binding site strongest corresponding sites from plant dehydratases that require Mg2+ activity. It differs respect to only one out 20 in pea ALAD.
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