Standardization and validation issues of the ELISPOT assay.
作者： Sylvia Janetzki , Josephine H. Cox , Neal Oden , Guido Ferrari
DOI: 10.1385/1-59259-903-6:051
关键词:
摘要: During the last 20 yr, enzyme-linked immunospot (ELISPOT) assay has emerged as one of most important and widely used assays to monitor immune responses in humans a variety other species. With ELISPOT assay, cell frequencies can be measured at single level without elaborate expansion or manipulation populations. Its usefulness led its application vaccine design development and, importantly, monitoring vaccination efforts. The impact results with this profound. In addition ease performance, repeatability reliability are major features expected an assay. focus today is on standardization technique, validation strategies comply these required features, accommodation growing demand Good Laboratory Practice (GLP) compliance. This chapter will give experienced scientists well newcomers field overview over issues for each step protocol. Guidelines given how validate performance.
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