Determination of the prevalence of Entamoeba histolytica and E. dispar in the pernambuco state of northeastern Brazil by a polymerase chain reaction.

摘要: Previous studies using methods varying from traditional serologic tests to molecular biology techniques have shown that in northeastern Brazil, Entamoeba dispar was more prevalent than E. histolytica. In this study, the prevalence established by histolytica stool antigen detection kits and a polymerase chain reaction (PCR) with genomic DNA extracted cultured trophozoites all four-nuclei, amoeba-positive samples population living Macaparana Brazil. Among 1,437 analyzed, only 59 (4.1%) were positive for four nuclei amoeba. However, of these negative an immunoenzymatic assay presence histolytica-specific galactose adhesin. Of cultivated samples, 31 showed trophozoites. Extraction followed PCR, 23 (74.19%) no amplification observed pathogenic The remaining eight both species. These findings are consistent those previously reported.