Identification of mannose 6-phosphate in two asparagine-linked sugar chains of recombinant transforming growth factor-beta 1 precursor.
作者： A F Purchio , J A Cooper , A M Brunner , M N Lioubin , L E Gentry
DOI: 10.1016/S0021-9258(18)68207-9
关键词:
摘要: Recombinant transforming growth factor-beta 1 (TGF-beta 1) precursor produced and secreted by a clone of Chinese hamster ovary cells was found to be glycosylated phosphorylated. Treatment 32P-labeled protein with N-glycanase indicated that phosphate incorporated into asparagine-linked complex carbohydrate moieties. Fractionation glycopeptides followed amino acid sequence analysis greater than 95% the label two out three glycosylation sites at Asn-82 Asn-136 TGF-beta precursor. Two-dimensional electrophoretic hydrolyzed protein-derived 32P as mannose 6-phosphate. Binding studies purified receptor for 6-phosphate could bind this binding specifically inhibited
sci-hub.st 参考文章(25)
A. Hasilik, E.F. Neufeld, BIOSYNTHESIS OF LYSOSOMAL ENZYMES IN FIBROBLASTS. SYNTHESIS AS PRECURSORS OF HIGHER MOLECULAR WEIGHT Journal of Biological Chemistry. ,vol. 255, pp. 4937- 4945 ,(1980) , 10.1016/S0021-9258(19)85585-0
R K Assoian, A Komoriya, C A Meyers, D M Miller, M B Sporn, Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization. Journal of Biological Chemistry. ,vol. 258, pp. 7155- 7160 ,(1983) , 10.1016/S0021-9258(18)32345-7
Erhard Gross, Bernhard Witkop, Nonenzymatic Cleavage of Peptide Bonds: The Methionine Residues in Bovine Pancreatic Ribonuclease Journal of Biological Chemistry. ,vol. 237, pp. 1856- 1860 ,(1962) , 10.1016/S0021-9258(19)73948-9
Complete amino acid sequence of human transforming growth factor type beta 2. Journal of Biological Chemistry. ,vol. 262, pp. 12127- 12131 ,(1987) , 10.1016/S0021-9258(18)45325-2
G G Sahagian, M M Gottesman, The predominant secreted protein of transformed murine fibroblasts carries the lysosomal mannose 6-phosphate recognition marker. Journal of Biological Chemistry. ,vol. 257, pp. 11145- 11150 ,(1982) , 10.1016/S0021-9258(18)33944-9
R Derynck, J A Jarrett, E Y Chen, D V Goeddel, The murine transforming growth factor-beta precursor. Journal of Biological Chemistry. ,vol. 261, pp. 4377- 4379 ,(1986) , 10.1016/S0021-9258(17)38511-3
Jonathan A. Cooper, Bartholomew M. Sefton, Tony Hunter, [42] Detection and quantification of phosphotyrosine in proteins Methods in Enzymology. ,vol. 99, pp. 387- 402 ,(1983) , 10.1016/0076-6879(83)99075-4
L E Gentry, N R Webb, G J Lim, A M Brunner, J E Ranchalis, D R Twardzik, M N Lioubin, H Marquardt, A F Purchio, Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells. Molecular and Cellular Biology. ,vol. 7, pp. 3418- 3427 ,(1987) , 10.1128/MCB.7.10.3418
KENNETH SHARPLES, GREGORY D. PLOWMAN, TIMOTHY M. ROSE, DANIEL R. TWARDZIK, A.F. PURCHIO, Cloning and Sequence Analysis of Simian Transforming Growth Factor-β cDNA DNA (Mary Ann Liebert, Inc.). ,vol. 6, pp. 239- 244 ,(1987) , 10.1089/DNA.1987.6.239
John P. Chamberlain, Fluorographic detection of radioactivity in polyacrylamide gols with the water-soluble fluor, sodium salicylate Analytical Biochemistry. ,vol. 98, pp. 132- 135 ,(1979) , 10.1016/0003-2697(79)90716-4