Inhibition of Yes-Associated Protein-1 (YAP1) Enhances the Response of Invasive Breast Cancer Cells to the Standard Therapy.

摘要: Purpose The deregulation of the Hippo pathway results in translocation ofYes-associated protein-1 (YAP1) to nucleus exert an oncogenic effect. This effect has been demonstrated several malignancies, yet, breast cancer (BC), it remains controversial. present study aimed investigate significance YAP1 expression BC, its relation stem cells (CSCs), and inhibition on tumor cell survival. Patients Methods We evaluated protein gene using immunohistochemistry (IHC) RT-qPCR FFPE tissue from normal cases. also studied association with CSC (OCT4, NANOG, SOX2) different clinicopathologic characteristics. Two BC lines (MCF7 MDA-MB-231) were exposed concentrations inhibitor "verteporfin" viability was subsequently assessed. Results mRNA higher compared (p-value=0.040) luminal tumors triple-negative (TNBC) (p-value= 0.017). Its significantly associated pluripotency markers (OCT4 NANOG) 0.030 0.035, respectively) resulted a significant reduction both MCF-7 MDA-MB-231 cells. nuclear by IHC, which signifies activation, more evident invasive carcinomas in-situ foci where limited cytoplasm. pretreatment their sensitization tamoxifen doxorubicin, respectively, decreased proliferation Conclusion Our imply that is highly expressed activated could represent possible novel therapeutic strategy should be further explored investigated improve outcome patients.