In Vivo Selection of Highly Metastatic Cells from Surgical Specimens of Different Primary Human Colon Carcinomas Implanted into Nude Mice

摘要: The purpose of these studies was to select and isolate cells with increased liver-metastasizing potential from heterogeneous primary human colon carcinomas (HCCs). Cells derived a HCC classified as Dukes' stage B2 were directly established in culture or injected into the subcutis, cecum, spleen nude mice. Progressively growing tumors excised, dissociated, culture. Subsequent implantation cecum mice, all four lines produced only few liver tumor foci. metastases expanded then mice provide source for further cycles selection. With each successive vivo selection cycle, metastatic ability isolated propagated increased. Four yielded cell very high efficiency In parallel using another D, we that highly Successive growth properties cells, albeit lesser extent than it did those HCC. produce not due simple trapping liver. distribution [125I] iododeoxyuridine-labeled revealed that, shortly after injection spleen, comparable number either low arrested differences between low- high-degree became apparent by 24 h and, 72 h, survived These results demonstrate hepatic metastasis is selective process mouse model can be useful isolating studying relevant host organ factors regulate pathogenesis metastasis.