Sperm specific antigen(s) in detergent extract of rhesus monkey spermatozoa

摘要: Sperm surface antigens were prepared by detergent extraction (Hyamine-Triton, Rohm and Haas Co., Philadelphia, PA) of spermatozoa from rhesus monkeys. Heterologous antisera against the extracts produced in female New Zealand White (NZW) rabbits hyperimmunization. The antiserum was absorbed initially with sperm-free monkey seminal plasma then lyophilized tissue homogenates liver, kidney, spleen, pancreas male unabsorbed at least three precipitin lines extract double immunodiffusion tests possessed sperm immobilization agglutination antibody activity. showed one line retained activity only. completely removed either epididymal or washed ejaculated spermatozoa. same results obtained ammonium-sulfate-precipitated immunoglobulin preparations. Immunoelectrophoresis revealed only precipitation preparation. It is concluded that Hyamine-Triton contains a component which can be characterized as sperm-specific immunoabsorption techniques apparently associated but not immobilization.