Theoretical prediction of the chemiluminescence behaviour of the ergot alkaloids. Direct flow injection chemiluminescence determination of ergotamine tartrate

摘要: Abstract The present manuscript is dealing with the application of molecular connectivity calculations to predict chemiluminescent behaviour ergot alkaloids when they react common strong oxidants in liquid phase. Twenty compounds were theoretically studied by means a discriminant equation formerly published, being 19 them predicted as high probability. Empirical confirmation chemiluminometric performed few soluble and commercially available alkaloids. On basis these results, new FIA-direct method proposed for determination presenting higher light emission, pharmaceutical ergotamine. based on oxidation drug K 3 [Fe(CN) 6 ] sodium hydroxide medium, at seventy degrees enhanced presence dioxane. calibration up 5.0 mg l −1 , revealed linear dynamic graph 0.5 mg l . reproducibility between days resulted rsd (in slope %) 8.9 ( n 5) repeatability %R.S.D. = 20) 1.9 0.6 0.3 4.0 mg l respectively. LOD (s/n 3) × 10 −7  mg l sample throughput 73 h influence foreign also tested optimised flow-assembly applied other family.