Capillary electrophoretic determination of DNA damage markers: content of 8-hydroxy-2'-deoxyguanosine and 8-nitroguanine in urine.
作者： Meng-Jie Li , Jun-Bo Zhang , Wen-Li Li , Qing-Cui Chu , Jian-Nong Ye
DOI: 10.1016/J.JCHROMB.2011.10.026
关键词:
摘要: A sensitive and low-cost analytical method has been developed to determine 8-hydroxy-2'-deoxyguanosine (8-OHdG) 8-nitroguanine (8-NO(2)Gua) based on capillary electrophoresis with amperometric detection (CE-AD) after solid phase extraction (SPE). Under optimized condition, these two markers were well separated from other components coexisting in urine, exhibiting a linear calibration over the concentration range of 0.1-50.0 μg/mL limits ranging 0.02 0.06 μg/mL. The relative standard deviations (RSDs) 0.1-2.1% for peak area, 0.1-1.5% migration time, respectively. average recovery RSD within 100.0-108.0% 0.1-1.7%, It was found that urinary contents 8-OHdG 8-NO(2)Gua cancer patients significantly higher than those healthy ones.
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