Isolation and characterization of malachite green-removing yeast from a traditional fermented fishery product

摘要: The screening of malachite green (MG)-degrading microorganisms was carried out using various sources, namely, fish farms and a traditional fermented fishery product in Myanmar Thailand. enrichment culture method performed MG-containing broth media, colonies that showed the decolorization MG on plate media were isolated as MG-degrading candidates. From results sequencing D1/D2 domain 26S rRNA gene, strain M3, representative candidates identified halotolerant yeast, Debaryomycesnepalensis. Liquid chromatography-tandem mass spectrometry (LC–MS/MS) analysis indicated during incubation this strain, concentration gradually decreased eventually reached undetectable levels. Conversely, leucomalachite (LMG) increased, final amount LMG estimated to be approximately 40 % initial amount. In addition, proton nuclear magnetic resonance spectroscopy (1H-NMR) also tautomer or other aromatic decomposition products not detected major component at end incubation. These suggest M3 removed changed 60 some metabolites than LMG.