Optimization Carotenoids Isolation Of The Waste Crude Palm Oil Using -Amylase, -Amylase, And Cellulase

摘要: Waste oil industry has a great potential use, as 77% yield are an untapped waste that still high economic value base for the oleochemical industry, one of which is β-carotene. The study was conducted to optimize extraction carotenoids from Crude Palm Oil (CPO) and Mill Effluent (POME) by column chromatography method with single enzyme : -amylase, -amylase, or cellulase its mixed. In addition, saponification reaction performed on CPO separate unsaponifiable fraction addition pretreatment. results showed help enzymes better than separation prossses using chromatography. - Amylase, may release matrix fibers other carbohydrates in POME waste. Optimum conditions -amylase obtained at 70C pH 6.0, - amylase 60 C 4.0, 40 7.0. While use mixture cellulose -amylase ratio 1 2 5.5 temperature o C yielded optimal -carotene. Extractions enzymatic methods palm were able isolate -carotene 0.19 % equivalent 1160 ppm. Keyword: -caroten, POME, chromatography,and extraction.