Non-aqueous capillary electrophoresis for simultaneous separation and determination of three major active components in traditional medicinal preparations.
作者： Cunhong Li , Jiyou Zhang , Anjia Chen , Jianping Xie , Xingguo Chen
DOI: 10.1002/BMC.459
关键词:
摘要: A simple and sensitive non-aqueous capillary electrophoresis method has been developed for simultaneous assay of three bioactive components (puerarin, daidzein wogonin) in traditional medicinal preparations the first time. Optimum separation analytes was obtained on a 47 cm × 75 µm i.d. using buffer system 20% acetonitrile, 25 mm ammonium acetate apparent pH 9.00, with applied voltage temperature 20 kV 16°C, respectively. The relative standard deviations (RSDs) migration times peak areas were ranges 2.5–4.0% 3.2–3.9%, Detection limits puerarin, wogonin 0.090, 0.145 0.090 µg mL−1, In tested concentration range, good linear relationships (correlation coefficients: 0.9998 0.9978 between concentrations observed. This successfully to determination recoveries from 91.0 114.0%. Copyright © 2004 John Wiley & Sons, Ltd.
sci-hub.se 参考文章(21)
Wing Ming Keung, Anti-dipsotropic isoflavones: the potential therapeutic agents for alcohol dependence. Medicinal Research Reviews. ,vol. 23, pp. 669- 696 ,(2003) , 10.1002/MED.10049
YOSHIO TAKINO, TAKETSUNE MIYAHARA, EIKO ARICHI, SHIGERU ARICHI, TERUAKI HAYASHI, MASAMI KARIKURA, Determination of some flavonoids in Scutellariae Radix by high-performance liquid chromatography Chemical & Pharmaceutical Bulletin. ,vol. 35, pp. 3494- 3497 ,(1987) , 10.1248/CPB.35.3494
Ara Kirakosyan, Peter B. Kaufman, Sara Warber, Steven Bolling, Soo Chul Chang, James A. Duke, Quantification of major isoflavonoids and l-canavanine in several organs of kudzu vine (Pueraria montana) and in starch samples derived from kudzu roots Plant Science. ,vol. 164, pp. 883- 888 ,(2003) , 10.1016/S0168-9452(03)00077-3
Gang Chen, Jianxia Zhang, Jiannong Ye, Determination of puerarin, daidzein and rutin in Pueraria lobata (Wild.) Ohwi by capillary electrophoresis with electrochemical detection Journal of Chromatography A. ,vol. 923, pp. 255- 262 ,(2001) , 10.1016/S0021-9673(01)00996-7
Hsi-Ya Huang, You-Zung Hsieh, Determination of puerarin, daidzein, paeoniflorin, cinnamic acid, glycyrrhizin, ephedrine, and [6]-gingerol in Ge-gen-tang by micellar electrokinetic chromatography Analytica Chimica Acta. ,vol. 351, pp. 49- 55 ,(1997) , 10.1016/S0003-2670(97)00349-8
Ying-Mei Liu, Shuenn-Jyi Sheu, Capillary electrophoresis determination of baicalein, baicalin, wogonin and wogonin-7-O-glucuronide in traditional Chinese medicinal preparations Journal of High Resolution Chromatography. ,vol. 17, pp. 559- 560 ,(1994) , 10.1002/JHRC.1240170713
Hee Kee Kim, Bong Sun Cheon, Young Ha Kim, Sung Yong Kim, Hyun Pyo Kim, Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochemical Pharmacology. ,vol. 58, pp. 759- 765 ,(1999) , 10.1016/S0006-2952(99)00160-4
Jeevan K. Prasain, Kenneth Jones, Marion Kirk, Landon Wilson, Michelle Smith-Johnson, Connie Weaver, Stephen Barnes, Profiling and quantification of isoflavonoids in kudzu dietary supplements by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry. Journal of Agricultural and Food Chemistry. ,vol. 51, pp. 4213- 4218 ,(2003) , 10.1021/JF030174A
Kwei-Lan Li, Shuenn-Jyi Sheu, Determination of flavonoids and alkaloids in the scute-coptis herb couple by capillary electrophoresis Analytica Chimica Acta. ,vol. 313, pp. 113- 120 ,(1995) , 10.1016/0003-2670(95)00190-B
Andreana L. Ososki, Edward J. Kennelly, Phytoestrogens: a review of the present state of research. Phytotherapy Research. ,vol. 17, pp. 845- 869 ,(2003) , 10.1002/PTR.1364