MicroRNA-148b and microRNA-152 reactivate tumor suppressor genes through suppression of DNA methyltransferase-1 gene in pancreatic cancer cell lines

摘要: Overexpression of DNA methyltransferase 1 (DNMT-1) is observed mostly in pancreatic cancer and it can cause tumor suppressor genes silencing this disease. Recent studies suggest that abnormal expressions microRNAs (miRs) are involved pathogenesis different types human cancers including cancer. In study we aimed to investigate the effect miR-148b -152 on reverting tumorigenic phenotype cell lines. In order whether regulation DNMT-1, luciferase reporter assay was used confirmed DNMT-1 mRNA could be a target for miR-152. Furthermore, overexpression lines (MIA PaCa-2 AsPC-1) decreased expression (53% 59% respectively), returned methylation normal patterns induced re-expression genes, like BNIP3 (4.7- 3.8-fold) SPARC (5.3- 2.9-fold) respectively. Moreover, introduced inhibit proliferation MIA (35% 37% respectively) AsPC-1 (39% 40% The apoptosis rates PaCa-1 after treatment with were 10% 8% respectively; while these 16% 11% Conclusively findings mean miRs targeting modifying status such as applied killing cells decreasing tumorigenicity cells.