Integration of Ecogpt and SV40 early region sequences into human chromosome 17: a dominant selection system in whole cell and microcell human-mouse hybrids.

摘要: The dominant selectable gene, Ecogpt, has been introduced, by the calcium phosphate precipitation technique, into normal human fibroblasts, along with SV40 early region genes. In one transfectant clone, integration of these sequences chromosome 17 was demonstrated construction human-mouse somatic cell hybrids, selected for growth in medium containing mycophenolic acid and xanthine. A whole hybrid, made between a mouse L cell, used as donor Ecogpt-carrying to 'tribrids' growing suspension, fusion thymoma line, microcell hybrids teratocarcinoma line. Two tribrids contained karyotypically chromosomes small number other chromosomes, while third tribrid had portion long arm translocated its only genetic material. independent no on background. These experiments demonstrate ability construct using selection system. By applying this approach it should be possible select wide range different hybrids. particular, transfer single cells will allow examination developmentally regulated gene after differentiation such