A one-plate assay for macrophage bactericidal activity.

摘要: Human peripheral blood monocytes purified by counterflow centrifugal elutriation (CCE) showed an enhanced capacity to kill Listeria Monocytogenes and Staphylococcus aureus after activation with interferon-gamma (IFN gamma). Bactericidal activity was evaluated a rapid colorimetric microassay based on the reduction of tetrazolium dye, MTT. The amount dye reduced directly proportional number viable bacteria present in each microwell. Comparison bacterial titration plate containing serial dilutions from stock known titer allowed calculation numbers before exposure macrophages. Results were read multiscan reader compared closely those obtained dilution agar plates (i.e., colony counts). entire assay, i.e., culturing monocytes, treatment IFN gamma, addition bacteria, quantitation surviving performed single microtiter plate.