Influence of chronic ethanol consumption on toxic effects of 1,2-dichloroethane: glycolipoprotein retention and impairment of dolichol concentration in rat liver microsomes and Golgi apparatus

摘要: Abstract Our previous investigations demonstrated that 1,2-dichloroethane (DCE) and chronic ethanol treatment separately are able to impair glycoprotein metabolism secretion, reduce dolichol concentration in liver membranes. The purpose of this study was investigate whether consumption can induce potentiation rat damage due DCE haloalkane used several chemical processes agriculture. Rats were given 36% their total energy as the Lieber–DeCarli liquid diet for 8 weeks (CH group). pair-fed control group received an isocaloric amount dextrine-maltose (PF ‘In vitro’ experiments: (6.5 mM) isolated hepatocytes from CH rats enhanced retention further reduced secretion 14 C-glucosamine incorporation compared or PF treated rats. vivo’ a marked decrease microsomes (in which dolichyl phosphate is rate-limiting initial glycosylation protein) Golgi membranes very important membrane permeability, fluidity vesicle fusion) observed acutely with 628 mg/kg bw (CH+DCE) PF+DCE These data suggest increases toxicity by affecting protein impairing glycolipoprotein concomitant at level apparatus.