Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
作者： Lia Maris Orth Ritter Antiqueira , Gabriel Dequigiovanni , Evandro Vagner Tambarussi , Jucelene Fernandes Rodrigues , Elizabeth Ann Veasey
DOI: 10.1590/1984-70332016V16N4N52
关键词:
摘要: Studies of genetic diversity and structure are key elements in designing effective situ ex management plans, especially for species experiencing forest fragmentation. To investigate the level populations Trichocentrum pumilum, eight polymorphic microsatellite loci were developed used genotyping 96 specimens from four disturbed populations. Low within was found (average number alleles per locus ranging 3.75 to 4.25, observed expected heterozygosities 0.238 0.333 0.450 0.482, respectively). The fixation index () ranged 0.35 0.47, with significant values all No genotypic disequilibrium detected. A mixed breeding system through an apparent outcrossing rate estimate. Our results suggest that these suitable studies this species, showing low population moderate T. pumilum
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