Octaheme nitrite reductase: The mechanism of intramolecular electron transfer and kinetics of nitrite bioelectroreduction
作者： Victor Andoralov , Sergey Shleev , Natalia Dergousova , Olga Kulikova , Vladimir Popov
DOI: 10.1016/J.BIOELECHEM.2020.107699
关键词:
摘要: Detailed impedance and voltammetric studies of hexameric octaheme nitrite reductase immobilized on carbon-based nanomaterials, specifically nanotubes nanoparticles, were performed. Well-pronounced bioelectrocatalytic reduction enzyme-modified electrodes was obtained. Analysis the data indicated absence long-lived intermediates involved in reduction. Cyclic voltammograms biomodified had a bi-sigmoidal shape, which pointed to presence two enzyme orientations carbon supports. The maximum (limiting) catalytic currents determined and, by applying correction mixed kinetics equation, Tafel dependences plotted for each wave/each orientation. Finally, schemes rate-limiting processes during bioelectrocatalysis proposed, viz. low- high-potential orientations.
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