Identification of two putative reference genes from grapevine suitable for gene expression analysis in berry and related tissues derived from RNA-Seq data
作者： Mauricio González-Agüero , Miguel García-Rojas , Alex Di Genova , José Correa , Alejandro Maass
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摘要: Background Data normalization is a key step in gene expression analysis by qPCR. Endogenous control genes are used to estimate variations and experimental errors occurring during sample preparation measurements. However, the transcription level of most commonly reference can vary considerably samples obtained from different individuals, tissues, developmental stages under variable physiological conditions, resulting misinterpretation performance target gene(s). This issue has been scarcely approached woody species such as grapevine.
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