Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays.

摘要: Identifying novel and known genes that are differentially expressed in breast cancer has important implications understanding the biology of tumorigenesis developing new diagnostic therapeutic agents. In this study we have combined two powerful technologies, PCR-based cDNA subtraction microarray, as a high throughput methodology designed to identify clones tumor- tissue-specific overexpressed tumors. Approximately 2000 generated from subtracted tumor library were arrayed on microarray chips. The target cDNAs then hybridized with 30 pairs fluorescent-labeled probes tumors normal tissues determine tissue distribution specificity. showing overexpression by further analysed DNA sequencing, GenBank EST database searches, quantitative real time PCR. We identified several genes, including mammaglobin, cytokeratin 19, fibronectin, hair-specific type II keratin, which previously been shown be may play an role malignance breast. also discovered B726P appears isoform NY-BR-1, gene. Two additional discovered, B709P GABAA receptor π subunit, not described for their profile Thus, combining allowed efficient way validate elevated mRNA expression levels potentially involved progression. These potential utility targets cancer.