RADYASYONUN SERUM DEMİR, BAKIR VE PROTEİN OKSİDASYON DÜZEYLERİNE ETKİSİ

作者： Özlem Gülbahar , Müge Akmansu , Aysel Arıcıoğlu , Zerrin Türközer

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摘要: Amac: Bir cok hastaligin etyopatogenezinde rol oynadigi dusunulen prote- inlerin oksidatif modifikasyonunu analiz etmek amaciyla en sik kullanilan yontem protein oksidasyon urunu olan karbonil gruplarinin tayinidir. Biz de calismamizda, radyasyonun serum uzerindeki etkisini gostermek grubu duzeylerini ve “metallerin katalizledigi oksidasyon” (metal-catalyzed oxidation, MCO) sistemleri ara- ciligiyla oksidasyonunu artirdigi bilinen demir (Fe) bakir (Cu) arastirmayi amacladik. Gerec Yontemler: Kobaylara radyasyon antioksidan (N-asetilsistein, NAC) uygulamalari sonrasinda Fe, Cu duzey- leri edildi. Protein duzeyi Levine metoduna gore, Fe du- zeyi otoanalizorde, ise atomik absorbsiyon cihazinda olculdu. Bulgular: Radyasyonun, grubu, duzeyle- rinde istatiksel olarak anlamli artislara neden oldugu goruldu. Radyasyon oncesinde NAC verilen grupta tek basina uygulanan gruba oranla stresin azaldigi gozlendi. yalnizca veril- mesi parametrelerde azalmaya oldu. Sonuc: Sonuc muhtemelen reaktif oksijen turlerinin zeyini artirarak, ayrica yukseltip metal katalizli oksi- dasyon yoluyla proteinlerin modifikasyonuna yol actigi, NAC’in olusturdugu oksidasyona karsi koruyucu bir etkisinin ol- dugu tespit Anahtar Kelimeler: Radyasyon, oksidasyonu, grubu. EFFECT OF RADIATION ON THE LEVELS IRON, COPPER AND PROTEIN OXIDATION IN SERUM Purpose: The method used most commonly to analyze oxidative modifica- tion of the proteins playing a role in etiopathogenesis various diseases is determination carbonyl groups, which are modificati- on products. To demonstrate effect radiation serum, we determined group levels that oxidation products and iron copper levels, known increase using metal-catalyzed (MCO) systems. Materials Methods: After exposure antioxidant (NAC), guinea pigs’ were anal- yzed. was by method, measured an autoanalyzer atomic absorption apparatus. Results: Radiation found significantly levels. Oxidative stress observed decrease given before compared receiving only. Giving only pigs led decreases pa- rameters. Conclusion: causes modification reactive oxygen radicals creating catalysis, has protective against due radiation. Key Words: Radiation, groups.

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RADYASYONUN SERUM DEMİR, BAKIR VE PROTEİN OKSİDASYON DÜZEYLERİNE ETKİSİ

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RADYASYONUN SERUM DEMİR, BAKIR VE PROTEİN OKSİDASYON DÜZEYLERİNE ETKİSİ

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