Polymerase Chain Reaction Diagnosis of Varicella Zoster Virus

摘要: Primary infection of humans by varicella zoster virus (VZV) results in chickenpox (varicella). Virus establishes latency sensory ganglia and reactivates decades later to produce shingles (zoster). Disseminated postherpetic neuralgia are the two major complications VZV reactivation elderly. There is increasing evidence that can reactivate subclinically (zoster without skin rash), particularly immunocompromised patients. Thus, clinical import rapid diagnosis use antiviral drugs these patients clear. Although most assays restricted their dependence on an adequate host immune response, limited sensitivity specificity, PCR technology enables detection minute amounts a specific viral DNA sequence large excess background DNA. The availability complete genome has facilitated during acute latent infections. already been detected isolated from human blood MNCs, throat swabs, vesicles crusts obtained at different times infection, latently infected ganglia. In addition, also used study early site replication mode transmission infection. Lastly, nested-primers quantitative will enhance permit determination burden biological specimens. These findings aid studies pathogenesis.