The Nuclear Microprobe - a Challenging Tool in Plant Sciences
作者： J. Mesjasz-Przybyłowicz
DOI: 10.12693/APHYSPOLA.100.659
关键词:
摘要: The nuclear microprobe is a microanalytical tool capable of quantitative studies elemental distribution at the ppm level with spatial resolution order 1 μm. This sensitivity adequate for most elements ofinterest, and acceptable in organs, tissues, cells. main techniques used plant science are particle induced X-ray emission using protons, proton backscattering, scanning transmission ion microscopy, gamma-ray emission. Specimen preparation difficult part analysis, only cryotechniques recommended presently all types studies.
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sci-hub.st 参考文章(27)
Oliver Strasser, Karin Köhl, Volker Römheld, Overestimation of apoplastic Fe in roots of soil grown plants Plant and Soil. ,vol. 210, pp. 179- 189 ,(1999) , 10.1023/A:1004650506592
U.B. Sleytr, Low Temperature Methods in Biological Electron Microscopy ,(1985)
Patrick Echlin, Low-temperature microscopy and analysis ,(1992)
D. B. Lazof, J. G. Goldsmith, T. W. Rufty, R. W. Linton, The early entry of Al into cells of intact soybean roots : A comparison of three developmental root regions using secondary ion mass spectrometry imaging Plant Physiology. ,vol. 112, pp. 1289- 1300 ,(1996) , 10.1104/PP.112.3.1289
G.J.F. Legge, A.P. Mazzolini, Elemental microanalysis of biological and medical specimens with a scanning proton microprobe Nuclear Instruments and Methods. ,vol. 168, pp. 563- 569 ,(1980) , 10.1016/0029-554X(80)91311-7
I.M. Weiersbye-Witkowski, W.J. Przybylowicz, C.J. Straker, J. Mesjasz-Przybylowicz, Elemental micro-PIXE mapping of hypersensitive lesions in Lagenaria sphaerica (Cucurbitaceae) resistant to Sphaerotheca fuliginea (powdery mildew) Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms. ,vol. 130, pp. 388- 395 ,(1997) , 10.1016/S0168-583X(97)00232-2
A. C. J. Timmers, H. -D. Reiss, J. Bohsung, K. Traxel, J. H. N. Schel, Localization of calcium during somatic embryogenesis of carrot (Daucus carota L.). Protoplasma. ,vol. 190, pp. 107- 118 ,(1996) , 10.1007/BF01281199
C.G. Ryan, D.N. Jamieson, C.L. Churms, J.V. Pilcher, A new method for on-line true-elemental imaging using PIXE and the proton microprobe Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. ,vol. 104, pp. 157- 165 ,(1995) , 10.1016/0168-583X(95)00404-1
C.G. Ryan, D.N. Jamieson, Dynamic analysis: on-line quantitative PIXE microanalysis and its use in overlap-resolved elemental mapping Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms. ,vol. 77, pp. 203- 214 ,(1993) , 10.1016/0168-583X(93)95545-G
Mischa Maetz, Arthur Schüßler, Alexandros Wallianos, Kurt Traxel, SUBCELLULAR TRACE ELEMENT DISTRIBUTION IN GEOSIPHON PYRIFORME Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms. ,vol. 150, pp. 200- 207 ,(1999) , 10.1016/S0168-583X(98)00929-X