A cellular gene encodes scrapie PrP 27-30 protein
作者： Bruno Oesch , David Westaway , Monika Wälchli , Michael P. McKinley , Stephen B.H. Kent
DOI: 10.1016/0092-8674(85)90333-2
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摘要: A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to N terminus protein. Southern blotting with revealed single gene same restriction patterns normal and DNA. PrP-related also detected murine human mRNA found at similar levels brain, as well many other tissues. Using antisera against crude extracts infected lesser extent brain. Proteinase K digestion yielded 27-30 extract, but completely degraded extract. No nucleic acids were prions, indicating that is not encoded acid carried within infectious particles.
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Stanley B. Prusiner, Prions: Novel Infectious Pathogens Advances in Virus Research. ,vol. 29, pp. 1- 56 ,(1984) , 10.1016/S0065-3527(08)60404-2
A. G. Dickinson, H. Fraser, Scrapie: Pathogenesis in Inbred Mice: An Assessment of Host Control and Response Involving Many Strains of Agent Springer, New York, NY. pp. 3- 14 ,(1977) , 10.1007/978-1-4612-9883-0_1
Erhard Gross, [27] The cyanogen bromide reaction Methods in Enzymology. ,vol. 11, pp. 238- 255 ,(1967) , 10.1016/S0076-6879(67)11029-X
M. KONDO, R. GALLERANI, C. WEISSMANN, Subunit structure of Q-beta replicase. Nature. ,vol. 228, pp. 525- 527 ,(1970) , 10.1038/228525A0
D C Bolton, R K Meyer, S B Prusiner, Scrapie PrP 27-30 is a sialoglycoprotein. Journal of Virology. ,vol. 53, pp. 596- 606 ,(1985) , 10.1128/JVI.53.2.596-606.1985
J.B. Olmsted, Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples. Journal of Biological Chemistry. ,vol. 256, pp. 11955- 11957 ,(1981) , 10.1016/S0021-9258(18)43211-5
R.M. Hewick, M.W. Hunkapiller, L.E. Hood, W.J. Dreyer, A gas-liquid solid phase peptide and protein sequenator. Journal of Biological Chemistry. ,vol. 256, pp. 7990- 7997 ,(1981) , 10.1016/S0021-9258(18)43377-7
P. E. Bendheim, J. M. Bockman, M. P. McKinley, D. T. Kingsbury, S. B. Prusiner, Scrapie and Creutzfeldt-Jakob disease prion proteins share physical properties and antigenic determinants Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 997- 1001 ,(1985) , 10.1073/PNAS.82.4.997
C. J. Gibbs, D. C. Gajdusek, R. Latarjet, Unusual resistance to ionizing radiation of the viruses of kuru, Creutzfeldt-Jakob disease, and scrapie. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 6268- 6270 ,(1978) , 10.1073/PNAS.75.12.6268
Stanley B. Prusiner, Michael P. McKinley, Karen A. Bowman, David C. Bolton, Paul E. Bendheim, Darlene F. Groth, George G. Glenner, Scrapie prions aggregate to form amyloid-like birefringent rods Cell. ,vol. 35, pp. 349- 358 ,(1983) , 10.1016/0092-8674(83)90168-X