Wnt3a upregulates transforming growth factor‐β‐stimulated VEGF synthesis in osteoblasts
作者： Hideo Natsume , Haruhiko Tokuda , Rie Matsushima-Nishiwaki , Kenji Kato , Kengo Yamakawa
DOI: 10.1002/CBF.1759
关键词:
摘要: It is recognized that Wnt3a affects bone metabolism via the canonical Wnt/β-catenin signalling pathway. We have previously shown transforming growth factor-β (TGF-β) stimulates synthesis of vascular endothelial factor (VEGF) p44/p42 mitogen-activated protein (MAP) kinase, stress-activated kinase (SAPK)/c-Jun N-terminal (JNK) and p38 MAP in osteoblast-like MC3T3-E1 cells. In present study, we investigated effect on TGF-β-stimulated VEGF these Wnt3a, which alone had little levels, significantly enhanced release. Lithium chloride SB216763, inhibitors glycogen synthase 3β, markedly amplified failed to affect TGF-β-induced phosphorylation Smad2, or SAPK/JNK. lithium strengthened mRNA expression induced by TGF-β. These results strongly suggest upregulates stimulated TGF-β activation pathway osteoblasts. Copyright © 2011 John Wiley & Sons, Ltd.
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