Detection of T-2 toxin by an improved radioimmunoassay.

摘要: T-2 toxin in serum, urine, and saline was analyzed by a modified radioimmunoassay procedure. The specimens were added directly to the assay tubes without extraction steps. reaction between antibody ligands optimal at 1 h. Albumin-coated charcoal used separate bound from free radioactivity. Quenching, which occurred with hemolyzed specimens, corrected wet oxidation process 60% perchloric acid 30% hydrogen peroxide. shorter incubation times resulted an that takes less than 6 h complete. average affinity constant of (Km) 1.75 X 10(10) liters/mol. sensitivity ng per or 10 ng/ml. Among other trichothecenes tested, only H-T-2 cross-reacted significantly (10.3%).