Comparison of conventional and molecular techniques for Trypanosoma vivax diagnosis in experimentally infected cattle

摘要: Livestock infections by Trypanosoma vivax have been occurring with increasing frequency, mainly due to the presence of animals subclinical and without apparent parasitaemia, making diagnosis challenging. The aim present study was evaluate several techniques used for T. in order assess best way using them during course disease. Molecular methods demonstrated higher rates detection than parasitological methods, detecting 33 54 (61.1%) known positive samples, while hematocrit centrifugation technique (best test) detected only 44.4%. serological IFAT ELISA, seropositivity 51 (94.4%) 49 (90.7%) respectively. Despite being highly sensitive, latter demonstrates exposure infectious agent does not indicate whether infection is active. first use qPCR a South American isolate, improving disease quantification. Furthermore, analyses revealed that patent phase may extend up 42 days, longer previously reported. combination diagnostic can lower frequency false negative results contributes toward better control.