Localization and Biological and Physicochemical Properties of the Cell Wall Lipopolysaccharide of Rhodopseudomonas capsulata

摘要: Electron micrographs of phenol-water-extracted lipopolysaccharide (LPS) Rhodopseudomonas capsulata show filamentous and netlike aggregates. Treatment the LPS with sodium deoxycholate resulted in a reversible splitting into subunits. The represents cell wall constituent O-antigenic specificity. In passive hemagglutination tests, high titers were obtained when erythrocytes sensitized untreated or heat-treated incubated antisera by immunization rabbits whole cells R. capsulata. alkali-treated was not active this test. Mouse lethality tests have shown that is less toxic than Escherichia coli. Also, X-ray protection efficacy phagocytic activity stimulation from mice are small, as compared E. Incubation living bacteria saline (37 C) solubilization an LPS-protein-lipid complex outer layer wall. isolated contained components which found LPS. addition, 20% amino acids large amount palmitic stearic acids, typical phospholipid components, present.