Reference gene validation for quantification of gene expression during ovarian development of turbot (Scophthalmus maximus).
作者： Yunhong Gao , Yuntao Gao , Bin Huang , Zhen Meng , Yudong Jia
DOI: 10.1038/S41598-020-57633-3
关键词:
摘要: Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is a powerful and sensitive method used in gene expression analysis. Suitable reference genes, which are stable under all experimental circumstances tissues significantly improve the accuracy of qRT-PCR data. In this study, stability six namely, 18S ribosomal RNA (18s), beta-actin (actb), elongation factor 1-alpha (ef1α), glyceraldehyde-3-phosphate-dehydrogenase (gapdh), cathepsin D (ctsd), beta-2-microglobulin (b2m) were evaluated as potential references for The genes examined hypothalamus-pituitary-ovary-liver (HPOL) axis throughout turbot ovarian development via using geNorm, NormFinder BestKeeper algorithms. Results showed that most ef1α, actb, ctsd hypothalamus, pituitary, ovary liver, respectively. best-suited combinations normalization 18s, hypothalamus; ctsd, 18s pituitary; ovary; gapdh liver. Moreover, profile estrogen receptor α (erα) manifested no significant difference to aforementioned during development. However, single or pair suitable an internal control account amplification differences among four summary, these results provide basic data optimal selection obtain highly accurate HPOL axis-related analysis
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